Oxidative stress and antioxidant enzyme activities in the trophocytes and fat cells of queen honeybees apis mellifera yushan hsieh 2 and chinyuan hsu 1, 2 1 department of biomedical. Study of genetic structure of honey bee from cyprus apis. We examined the influence of caffeine on honeybee lifespan, nosema resistance, key enzyme activities, metabolic compound concentrations, and total dna methylation levels. Apr, 2017 substantial percentage of world food production depends on pollinating service of honeybees that directly depends on their health status. The superoxide dismutase and catalase play an important role in protecting worker bees from reactive oxygen species, and their enzyme activities and expression levels are associated with honeybee longevity and disease resistance which affect the performance of the colony. Feb 23, 20 acacia honey is produced by apis mellifera, a cultured bee that harvests the extrafloral nectar of acacia mangium trees. This behaviour is the link between the honey bee colony and the ambient environment. Sister worker honeybees apis mellifera produced by a single queen bee were sampled at the beginning november 2010, approximately 6 weeks old and in the middle of winter january 2011, approximately 12 weeks old from one colony at the university apiary clujnapoca. Nov 21, 2017 the western honey bee apis mellifera is a eusocial species whose foragers collect food to meet hive requirements and adjust their foodgathering behavior according to these collective needs. However, the higher temperatures and humidity in greenhouses are not conducive to the survival of bees. Honey bee antioxidant enzymes are of particular interest because of their potential.
Pdf antioxidant enzymes in the honey bee, apis mellifera. Tolerance and response of two honeybee species apis. The honey bee apis mellifera, the test species used in the current environmental risk assessment procedure, is generally considered as extremely sensitive to pesticides when compared to other bee. The substantial activities of all three enzymes in spermathecae of mated queens suggest their involvement in the longterm protection of the spermatozoa from oxidative stress. It is the only insect that produces food eaten by man. Pineapple honey is also produced by the apis mellifera bees that collect the nectar of pineapple ananas comosus flowers, resulting in a honey with a distinctive aroma and flavor. Gut pathology and responses to the microsporidium nosema.
Honey is nectar collected from many plants and processed by honey bees apis mellifera. Supplementing with vitamin c the diet of honeybees apis mellifera carnica parasitized with varroa destructor. The basic biology of apis mellifera, the western honeybee 3. Nontarget inhibition of antioxidant enzymes in honey bees a.
Antioxidant activity and irritation property of venoms. Antioxidant enzymes in the honey bee, apis mellifera gunter weirich, anita collins, virginia williams to cite this version. Evans usdaars bee research laboratory, beltsville, md, usa abstract honey bee apis mellifera. They play a crucial role in the detoxification of exogenous compounds, especially. Honey bees collect floral nectar and pollens, for feeding, which.
Honey bee apis mellifera sperm remains viable in the spermatheca of mated female honey bees for several years. Transcriptomic analysis reveals apis mellifera adaptations. Classification of the protein sequences showing decreased expression p apis mellifera exposed to fieldrelevant doses of the fungicide pyraclostrobin and insecticide. The protective strategy of antioxidant enzymes against. Although this parasite is presently spreading across the world into its novel host, the mechanisms by it which affects the bees and how bees respond are not well understood.
Request pdf toxicity of oxalic acid and impact on some antioxidant enzymes on in vitroreared honeybee larvae nowadays, varroa destructor is considered as a serious pest of honeybees apis. Nutritional effect of alphalinolenic acid on honey bee. The protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, apis mellifera during two different seasons by ahmad m. Unexpectedly strong effect of caffeine on the vitality of.
Nontarget inhibition of antioxidant enzymes in honey bees. In this study, we assayed reactive oxygen species ros and antioxidant enzymes. The aim of this study was to evaluate the antibacterial effects of ethiopian honeys against antibiotic susceptible and. Evans usdaars bee research laboratory, beltsville, md, usa abstract honey bee apis mellifera sperm remains viable in the spermatheca of mated female honey bees for several years. Physicochemical and antioxidant properties of malaysian. To develop a fundamental and comprehensive understanding of lipids in rj, this. Apis mellifera are important pollinating bees that are widely used in agricultural systems. Both these enzymes are part of the primary antioxidant response and act. Terrestrial ecosystems are exposed to various kinds of pollutants, including radionuclides. Foraging behaviour is one of the distinctive behaviours of honey bees, apis mellifera. Flumethrin at honeyrelevant levels induces physiological.
Sperm storage and antioxidative enzyme expression in the honey bee, apis mellifera. Pdf the protective strategy of antioxidant enzymes against. Accepted, 304 accepted, 304 a gricultural r esearch s ervice, b eltsville a rea b ee r esearch l aboratory. Honey bees, apis mellifera, are markedly less sensitive to neonicotinoid insecticides containing a cyanoimino pharmacophore than to those with a nitroimino group.
Using mitochondria isolated from the thoraces, complete oxidation of most of the tca cycle members has been shown. Antioxidative enzymes might help reduce this damage. To understand how the physiological and cellular mechanisms that determine the onset and duration of senescence are shaped by behavioral development and behavioral duration, we exploited the tractability of the honey bee apis mellifera model system. Chemical communication in the honeybee apis mellifera l. Newly emerged worker bees were exposed to organophosphate insecticide coumaphos, a neonicotinoid imidacloprid, and their mixtures. Toxicity of oxalic acid and impact on some antioxidant. We determined that constituents found in honey, including p coumaric acid, pinocembrin, and pinobanksin 5methyl ether. What is the main driver of ageing in longlived winter. The foraging behaviour of honey bees, apis mellifera. Honey bee apis mellifera drones survive oxidative stress due to increased tolerance instead of avoidance or repair of oxidative damage hongmei libyarlaya,b,c. Frequent and highly aerobic behaviors likely contribute to naturally occurring stress, accelerate senescence and limit lifespan.
In this study, we assayed reactive oxygen species ros and antioxidant enzymes in the trophocytes and fat cells of young and old queens. The objective of this study was to record the use and to evaluate the antioxidant, antihyperglycemic, and antidiabetic activity of apis mellifera. Honey bees, scientifically also known as apis mellifera, which mean honeycarrying bee, are environmentally friendly and are vital as pollinators. Moreover this organisation has been surprisingly little altered by the \domestication of the bees. The protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, apis mellifera during two different seasons article pdf available january 2012 with 228 reads how we. In the present study, honeybees were irradiated right after birth for 14 days with gamma rays at dose rates ranging between 4.
Nowadays, varroa destructor is considered as a serious pest of honeybees apis mellifera and its resistance to acaricides has been reported in europe since the early 1990s. Understanding how different insecticides influence bee health is very essential. Antibacterial effects of apis mellifera and stingless bees. The honeybee, apis mellifera, is commonly used in ecotoxicology as a model species for evaluating the effects of pollutants. Induced thiacloprid insensitivity in honeybees apis. May 14, 2019 korayem am, khodairy mm, abdelaal aaa, elsonbaty aam 2012 the protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, apis mellifera during two different seasons. Sperm storage and antioxidative enzyme expression in the. The protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, apis mellifera during two different seasons. Honeybee antioxidant enzymes are of particular interest because of their.
This study was to observe the developmental regulation of cuznsod, mnsod and cat genes in apis mellifera. Pdf the protective strategy of antioxidant enzymes. Behavioral responses of honey bees apis mellifera to. Transcriptomic analysis reveals apis mellifera adaptations to high temperature and high humidity. Glutathione stransferases gsts are an important family of multifunctional enzymes in aerobic organisms. The most important antioxidant enzymes identified in honeybee body fluids are superoxide dismutase sod, catalase cat, glutathione. We also evaluated the changes in the activity of antioxidant and detoxification enzymes. Curcumin stimulates biochemical mechanisms of apis. Formetanate toxicity and changes in antioxidant enzyme system of apis mellifera larvae. The species apis mellifera, belonging to the subfamily apinae, known as the european honey bee, is exotic in brazil and is a major producer of propolis, which has been reported to present important antioxidant, antimicrobial 14,29 and antitumour 30,31 activities. Honeybee apis mellifera royal jelly rj has a long history in human medicine because of its healthprotecting properties. Request pdf sperm storage and antioxidative enzyme expression in the honey bee, apis mellifera honey bee apis mellifera sperm remains viable in the spermatheca of mated female honey bees for. Trophocytes and fat cells of queen honeybees have been used for delayed cellular senescence studies, but their oxidative stress and antioxidant enzyme activities with advancing age are unknown. Insects have a range of antioxidant enzymes, mainly composed of superoxide dismutase sod, catalase cat, and peroxidase pod.
Formetanate toxicity and changes in antioxidant enzyme system. The basic biology of apis mellifera the western honeybee. Among other factors, the success of bee colonies depends on health of developed larvae. Total antioxidant capacity of honeybee haemolymph in relation to. Honey bee rearing, collection and dissection honey bees apis mellifera linnaeus 1758 for this study were reared at the university of nevada, las vegas unlv, nv, usa apiary. Curcumin stimulates biochemical mechanisms of apis mellifera. Antioxidant properties of honey and its role in preventing. Several physical, biochemical and antioxidant properties of two nigerian stingless bee honey varieties melipona sp. The objective of this study was to research the effect of six dietary ala levels on the growth and development of apis mellifera ligustica colonies. Although previous work has suggested that this results from enhanced metabolism of the former by detoxification enzymes, the specific enzyme. Honey constituents upregulate detoxification and immunity. Various detoxifying enzymes, including microsomal oxidases, glutathione stransferases, esterases, epoxide hydrolase, and ddtdehydrochlorinase, were assayed in adult worker bees apis mellifera l.
The genus name apis is latin for bee, and mellifera is the latin for honey. Foraging and pollination activity of apis mellifera adansonii. Oct 19, 20 when apis mellifera honey eucalyptus and stingless bees honey were compared, the former had higher phenolic and flavonoid contents than the stingless bees honey, which in turn had the higher antioxidant activity. The western honey bee or european honey bee apis mellifera is the most common of the 712 species of honey bees worldwide. The honey bee apis mellifera is an ecologically and. Recent declines in bee populations have prompted numerous studies on the. Apis mellifera bee tea is used by indigenous brazilians to treat diabetes, and this traditional knowledge needs to be recorded and studied. Supplementing with vitamin c the diet of honeybees apis.
Differential expression of mitochondrial genes between queens and workers during caste determination in the honeybee apis mellifera. Evaluation of physicochemical and antioxidant properties of. Although modern dictionaries may refer to apis as either honey bee or honeybee, entomologist robert snodgrass asserts that correct usage requires two words, i. The honey bee apis mellifera is an ecologically and economically important pollinator species worldwide. Effects of flight activity and age on oxidative damage in the honey. For the honey bee apis mellifera the assessment of antioxidative capability became easier after the sequencing of the honey bee genome. Apis mellifera, apis cerena, apis florea, and apis dorsata.
This natural product is widely appreciated as the only concentrated form of sugar available. In this study, insecticide incidence, dna damage, and antioxidant enzyme activity were studied intrareas. High levels of these indicators ensure a high level of antioxidants in honey. Contribution of lipids in honeybee apis mellifera royal. Changes in mrna expression level of antioxidant enzyme. The difference in floral sources, in these two localities, might explain the difference in antibacterial effects of apis mellifera tigray white and gondar yellow honeys. Tested enzymes showed variable affinity to substrates. Three honey bee samples s, of 30 g honey bees each, were collected from each sz for the honey bee species a. Supplementation of the honey bee diet with vitamin c. Antioxidant enzymes in the honey bee, apis mellifera. The main objective of this study was to test comparatively the effects of two common insecticides on honey bee apis mellifera workers lifespan, food consumption, mortality, and expression of antioxidant genes. Antioxidant enzymes in the honey bee, apis mellifera apidologie. Effects of flight activity and age on oxidative damage in. Archive ouverte hal antioxidant enzymes in the honey bee, apis mellifera.
Sperm storage and antioxidative enzyme expression in the honey bee, apis mellifera a. May 28, 20 as a managed pollinator, the honey bee apis mellifera is critical to the american agricultural enterprise. Each colony contained a young normal egglaying queen and had a working population of eight frames of comb with larvae, pupae, honey, and pollen dai et al. Catalase cat, glutathione stransferase gst and superoxide dismutase sod activities were determined in postmitochondrial fractions of. Alphalinolenic acid ala, which is an n3 polyunsaturated fatty acid pufa, influences honey bee feed intake and longevity. Antioxidant enzymes in the honey bee, apis mellifera article pdf available in apidologie 331 january 2002 with 918 reads how we measure reads. The honeybee apis mellifera belongs to the hymenoptera insects that live in social groups with a fixed function of individuals including their rotation during the ontogeny of individuals, resulting in a swarm, which works to some extent, as an organism. Effects of coumaphos and imidacloprid on honey bee. Mic of white and yellow honeys for 40% of the test organisms was 6. Oxidative stress and antioxidant enzyme activities in the.
Based on this, corona and robinson 2006 showed at least 38 genes coding for antioxidative proteins. Modification of the head proteome of nurse honeybees apis. Acacia and pineapple honey are produced by apis mellifera bees while borneo and tualang honey are produced by apis cerana and apis dorsata bees, respectively. Antioxidant properties of honey act as an antidepressant during high emotional, physical and intellectual stress 17. Substantial percentage of world food production depends on pollinating service of honeybees that directly depends on their health status. In this study, we explored the effects of higher temperature and humidity on the epiphysiology of bees by evaluating the survival, tolerance and body water loss in two bee species apis cerana and apis mellifera. The crucial phase of larval development is the first 6 days after hatching when a worker larva grows exponentially and larvae are potentially exposed to xenobiotics via diet. Bee venom bv can be produced by all species of honey bees, e. Honey is the only food that includes all the substances necessary to sustain life, including enzymes. The microsporidium nosema ceranae is a newly prevalent parasite of the european honey bee apis mellifera. Apis mellifera adansonii, nectar, pollen, allium cepa, pollination, maroua, abstract to evaluate the impact of apis mellifera adansonii hymenoptera. Abstract catalase cat, glutathionestransferase gst and superoxide dismutase sod activi ties were determined in postmitochondrial. Oxidative dissimilation has been studied in enzymes from the honey bee.
Physiological effects of gamma irradiation in the honeybee. Antioxidant, antihyperglycemic, and antidiabetic activity. Of the two species, the honeybee has by far the more elaborately organised life. Detoxification mechanisms of honey bees apis mellifera. We used sister worker offspring of a single queen to minimize genetic. Aboushaara faculty of agriculture, damanhour university, damanhour, egypt abstract. Honey bee apis mellifera drones survive oxidative stress.
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